Detection and Genotyping of Epstein-Bar Virus Among Paraffin Embedded Tissues of Hodgkin and Non-Hodgkin's Lymphoma Patients in Ahvaz, Iran

Ala Habibian; Manoochehr Makvandi; Alireza Samarbaf-Zadeh; Niloofar Neisi; Rahim Soleimani-Jelodar; Kamyar Makvandi; Shima Izadi

Acta Medica Iranica (Aug 2018)

Detection and Genotyping of Epstein-Bar Virus Among Paraffin Embedded Tissues of Hodgkin and Non-Hodgkin's Lymphoma Patients in Ahvaz, Iran

Ala Habibian,
Manoochehr Makvandi,
Alireza Samarbaf-Zadeh,
Niloofar Neisi,
Rahim Soleimani-Jelodar,
Kamyar Makvandi,
Shima Izadi

Affiliations

Ala Habibian: Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Manoochehr Makvandi: Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Alireza Samarbaf-Zadeh: Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Niloofar Neisi: Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Rahim Soleimani-Jelodar: Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Kamyar Makvandi: Department of Orthopedic Surgery, Taleghani Hospital Research Development Unit, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Shima Izadi: Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran.

Journal volume & issue: Vol. 56, no. 7

Abstract

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Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infected with 90% of the world’s population. EBV is transmitted through saliva and associated with different diseases such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitt`s lymphoma, Hodgkin and Non-Hodgkin`s lymphoma. This study was designed to determine the frequency of EBV DNA and genotyping of this virus in histological tissues of Hodgkin and Non-Hodgkin's lymphoma in Ahvaz, Iran. In this study, 12 samples of Hodgkin and 29 samples of Non-Hodgkin`s lymphoma were examined from Ahvaz Imam Khomeini Hospital. After deparaffinization and DNA extraction, Nested-PCR technique was carried out on Epstein-Barr nuclear antigen 1 (EBNA-1) and Epstein-Barr nuclear antigen 3C (EBNA-3C) regions in order to detect EBV genome and genotype in tumoral tissues. Among 12 cases of Hodgkin`s lymphoma, 3 (25%) cases and among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive for EBV. All 3 EBV positive cases of Hodgkin`s lymphoma and a number of 8 (57%) cases of Non-Hodgkin`s lymphoma belonged to the adolescence age group which proves an association between age and EBV positive in Hodgkin and Non-Hodgkin`s lymphoma according to Fisher`s exact test (P=0.03). All EBV positive cases in Hodgkin and Non-Hodgkin`s lymphoma were EBV-1. The results of this study revealed that EBV played a possible important role in Hodgkin and Non-Hodgkin`s lymphoma especially in adults. Also, the main genotype of virus in the current study was EBV-1.

Published in Acta Medica Iranica

ISSN: 0044-6025 (Print); 1735-9694 (Online)
Publisher: Tehran University of Medical Sciences
Country of publisher: Iran, Islamic Republic of
LCC subjects: Medicine: Medicine (General)
Website: http://acta.tums.ac.ir/

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