Overexpression of BjuJAZ8 from tuber mustard enhanced the sensitivity of Arabidopsis thaliana to salt stress

Abstract

[Objective] Tuber mustard has high economic value. Investigating the function of the jasmonic acid signaling pathway gene JAZ8 in response to salt stress can provide genetic resources for breeding saltresistant varieties of tuber mustard. [Methods] The gene structure, promoter cis-acting elements, and the physical and chemical properties of JAZ8 were analyzed by bioinformatics methods. JAZ8 gene was cloned and the overexpression vector was constructed. The JAZ8 gene of tuber mustard was overexpressed in the model plant Arabidopsis thaliana and the function of JAZ8 gene in Arabidopsis in response to salt stress was analyzed. [Results] The JAZ8 gene coding sequence was 396 bp, encoding 131 amino acids and containing TIFY and CCT-2 domains. The gene promoter contained cis-acting elements that respond to abscisic acid, salicylic acid, and participate in defense and stress response. Phenotypic analysis of the transgenic plants showed that Arabidopsis plants overexpressing BjuJAZ8 showed a more sensitive phenotype to salt stress during germination and greening. [Conclusion] Overexpression of JAZ8 from tuber mustard enhanced the sensitivity of Arabidopsis to salt stress.

Keywords

• tuber mustard
• bjujaz8
• salt stress
• gene function