Variations of total protein content and protein synthesis during microsporogenesis in Larix europaea L.

Abstract

The variations of the total protein content and protein synthesis were analyzed in successive stages of microsporogenesis in Larix europaea L. Pure fractions of microsporocytes containing the cells in successive phases of meiosis and fractions of the anther wall cells were used to perform the assays. It was revealed, that the protein content and the intensity of protein synthesis undergo considerable changes in the course of meiosis. It was proved also, by means of electrophoretic separation of protein fractions, that no new proteins are synthesised during differentiation of microsporocytes of larch, and that the microsporogenesis process is related rather to disappearance of some protein fractions.