ACTION OF PORPHYRINES BISMUTH COMPLEXES AND BACTERIOPHAGE ON PSEUDOMONAS AERUGINOSA BIOFILM FORMATION AND PIOCYANIN BIOSYNTHESIS

Abstract

It was shown that P. aeruginosa bacteriophage can posses inhibitory action on the quorum sensing system in presence of synthetic porphyrines bismuth complexes. Synthetic porphyrines bismuth complexes, used alone, show an ability to inhibit piocyanin biosynthesis depend of its concentration in culture media. Ві(III)-ТPP shows the highest inhibitory activity, Ві(III)-ПП IХ – the lowest. In presence of 0.4 μM Ві(III)-ТPP piocyanin concentration in culture media decreases 1.8 times as compared to the control. In presence of 40 μM Ві(III)-ТPP pigment concentration decreased 2.3 times and in presence of 80 μM – 3.1 times. In presence of bacteriophage (2½105 PFU/ml) and Ві(III)-ТPP concentrations (0.4; 4 and 80 μМ) pigment contain decreased 2.5; 4.7 and 10 times respectively. The biofilm mass on the synergistic action of the bacteriophage and Ві(III)-ТPP was three times lower than in presence of this porphyrine bismuth complex alone. Bismuth complexes of other porphyrines used, show the same effects, but rather lower than Ві(III)-ТPP. Bacteriophage used alone show no effects on the biofilm formation, while piocyanine biosynthesis in presence of bacteriophage strongly decreased.

Keywords

• біоплівка
• піоцианін
• p. aeruginosa
• бактеріофаг вісмутові комплекси порфіринів.