Development of a bench-top extra-cleanroom for DNA amplification
Hirokazu Takahashi,
Takahiro Satoh,
Hiroko Kanahara,
Yuji Kubota,
Tamaki Hirose,
Hiroyuki Yamazaki,
Kimiko Yamamoto,
Yoshiko Okamura,
Taketo Suzuki,
Toshiro Kobori
Affiliations
Hirokazu Takahashi
1Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan
Takahiro Satoh
4Environment Engineering Division, Koken Ltd, Tokyo, Japan
Hiroko Kanahara
3NanoBiotetcnology Laboratory, Food Engineering Division, National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan
Yuji Kubota
5Hanno Laboratory, Koken Ltd, Saitama, Japan
Tamaki Hirose
3NanoBiotetcnology Laboratory, Food Engineering Division, National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan
Hiroyuki Yamazaki
6Isehara Research Laboratory, Technology & Development Division, Kanto Chemical Co., Inc, Isehara, Kanagawa, Japan
Kimiko Yamamoto
7Insect Genome Laboratory, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan
Yoshiko Okamura
1Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan
Taketo Suzuki
4Environment Engineering Division, Koken Ltd, Tokyo, Japan
Toshiro Kobori
3NanoBiotetcnology Laboratory, Food Engineering Division, National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki, Japan
Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.
