Genetics and Molecular Biology ()

[PROVISIONAL] Perforin Gene PRF1 c.900C>T Polymorphism and HIV-1 Vertical Transmission

  • Luisa Zupin,
  • Vania Polesello,
  • Anselmo Jiro Kamada,
  • Rossella Gratton,
  • Ludovica Segat,
  • Louise Kuhn,
  • Sergio Crovella

DOI
https://doi.org/10.1590/1678-4685-gmb-2018-0243
Journal volume & issue
no. 0

Abstract

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Abstract Perforin-1, component of the immune system, is able to control Human Immunodeficiency Virus-1 (HIV-1) replication and it could be involved in HIV-1 mother-to-child transmission (MTCT). This study aims at evaluating the role of c.900C>T PRF1 gene (encoding for perforin-1) polymorphism (rs885822) in HIV-1 MTCT. PRF1 c.900C>T polymorphism was genotyped in 331 children from Zambia using Taqman probe on Real Time PCR platform. PRF1 c.900C>T C/T genotype was more frequent amongst HIV-1 exposed but non-infected children than in HIV-1 positive cases and the results were confirmed among children infected during breastfeeding. PRF1 c.900C>T correlated with protection against HIV-1 MTCT, suggesting its role in HIV-1 vertical transmission.

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