Generation of a human iPS cell line (CABi003-A) from a patient with age-related macular degeneration carrying the CFH Y402H polymorphism

Ana B. Garcia-Delgado; Sofia M. Calado; Lourdes M. Valdes-Sanchez; Adoracion Montero-Sanchez; Beatriz Ponte-Zuñiga; Berta de la Cerda; Shom Shanker Bhattacharya; Francisco J. Diaz-Corrales

Stem Cell Research (Jul 2019)

Generation of a human iPS cell line (CABi003-A) from a patient with age-related macular degeneration carrying the CFH Y402H polymorphism

Ana B. Garcia-Delgado,
Sofia M. Calado,
Lourdes M. Valdes-Sanchez,
Adoracion Montero-Sanchez,
Beatriz Ponte-Zuñiga,
Berta de la Cerda,
Shom Shanker Bhattacharya,
Francisco J. Diaz-Corrales

Affiliations

Ana B. Garcia-Delgado: Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain
Sofia M. Calado: Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain
Lourdes M. Valdes-Sanchez: Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain
Adoracion Montero-Sanchez: Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain
Beatriz Ponte-Zuñiga: Macarena University Hospital, Department of Ophthalmology, Seville, Spain; RETICS Oftared, Carlos III Institute of Health (Spain), Ministry of Health RD16/0008/0010, Seville, Spain
Berta de la Cerda: Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain
Shom Shanker Bhattacharya: Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain
Francisco J. Diaz-Corrales: Department of Regeneration and Cell Therapy, Andalusian Molecular Biology and Regenerative Medicine Centre-CABIMER (Junta de Andalucía, CSIC, Universidad de Sevilla, Universidad Pablo de Olavide), Seville, Spain; Corresponding author at: Andalusian Molecular Biology and Regenerative Medicine Centre, Avda. Americo Vespucio N°24 Edif. CABIMER, Parque Científico y Tecnológico Cartuja, 41092 Sevilla, Spain.

Journal volume & issue: Vol. 38

Abstract

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Age-related macular degeneration (AMD) is the leading cause of adult blindness in developed countries and is characterized by progressive degeneration of the macula, the central region of the retina. A human induced pluripotent stem cell (hiPSC) line was derived from peripheral blood mononuclear cells (PBMCs) from a patient with a clinical diagnosis of dry AMD carrying the CFH Y402H polymorphism. Sendai virus was using for reprogramming and the pluripotent and differentiation capacity of the cells were assessed by immunocytochemistry and RT-PCR.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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