Virology Journal (Sep 2006)

Genetic analysis of Thailand hantavirus in <it>Bandicota indica </it>trapped in Thailand

  • Hugot Jean-Pierre,
  • Plyusnina Angelina,
  • Herbreteau Vincent,
  • Nemirov Kirill,
  • Laakkonen Juha,
  • Lundkvist Åke,
  • Supputamongkol Yupin,
  • Henttonen Heikki,
  • Plyusnin Alexander

DOI
https://doi.org/10.1186/1743-422X-3-72
Journal volume & issue
Vol. 3, no. 1
p. 72

Abstract

Read online

Abstract Sixty one tissue samples from several rodent species trapped in five provinces of Thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. Four samples, all from the great bandicoot rat Bandicota indica, were confirmed positive for the hantaviral N-antigen. Two of them were trapped in Nakhon Pathom province, the other two in Nakhon Ratchasima province, approximately 250 km from the other trapping site. When analysed by RT-nested PCR, all four rodents were found positive for the hantaviral S- and M-segment nucleotide sequences. Genetic analysis revealed that the four newly described wild-type strains belong to Thailand hantavirus. On the phylogenetic trees they formed a well-supported cluster within the group of Murinae-associated hantaviruses and shared a recent common ancestor with Seoul virus.