Brazilian Archives of Biology and Technology (Feb 2014)

The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5

  • Francisco Esmaile de Sales Lima,
  • Helena Beatriz Ruthner Batista,
  • Fabrício Souza Campos,
  • Hiran Castagnino Kunert Filho,
  • Samuel Cibulski,
  • Fernando Finoketti,
  • Thalita Arantes,
  • Fernando Spilki,
  • Paulo Michel Roehe,
  • Franciscus Antonius Maria Rijsewijk,
  • Ana Cláudia Franco

DOI
https://doi.org/10.1590/S1516-89132014000100007
Journal volume & issue
Vol. 57, no. 1
pp. 45 – 47

Abstract

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This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells.

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