Dietary composition influences sperm quality and testis damage via endoplasmic reticulum stress in lambs

Abstract

Abstract Background The metabolic impacts of including soya meal, wheat gluten and corn gluten in the diet of male lambs could influence their reproductive performance. Objectives An experiment was carried out to assess the effects of corn gluten, wheat gluten and soya meal on the reproductive system of male lambs. Methods Twenty‐four male Morkaraman lambs, aged 9 months, were utilized in this study and were fed experimental diets for 56 days. The lambs were divided into a control group (soybean meal + safflower meal), a corn group (corn gluten) and a wheat group (wheat gluten). Results The serum follicle‐stimulating hormone level of the control group was significantly higher and tumour necrosis factor‐alpha (TNF‐α) level was lower than the wheat and corn gluten groups (p < 0.05). The lowest malondialdehyde level in testicular tissue was observed in the control group, whereas the highest was in the wheat gluten group (p < 0.05). The glutathione level in the control group was significantly higher than in the other groups (p < 0.05). The corn gluten group showed the highest CHOP and IRE1 levels; the lowest Bcl‐2 levels and the highest IL‐1B and P2 × 7R levels were found in the wheat group; and the lowest TNF‐α levels were in the control group (p < 0.05). Additionally, the study revealed that diet had a significant impact on spermatological parameters of the testis such as diameter, volume and weight (p < 0.05). Conclusions These results concluded that the inclusion of different protein sources in the diet of reproductive male lambs affects the metabolism of testicular tissue.

Keywords

• antioxidant
• gluten
• protein expression
• sperm quality