A lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria

Sangam Srikanth; U.S. Jayapiriya; Satish Kumar Dubey; Arshad Javed; Sanket Goel

iScience (Nov 2022)

A lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria

Sangam Srikanth,
U.S. Jayapiriya,
Satish Kumar Dubey,
Arshad Javed,
Sanket Goel

Affiliations

Sangam Srikanth: Department of Mechanical Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India
U.S. Jayapiriya: Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India
Satish Kumar Dubey: Department of Mechanical Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India
Arshad Javed: Department of Mechanical Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India
Sanket Goel: Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; Corresponding author

Journal volume & issue: Vol. 25, no. 11
p. 105388

Abstract

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Summary: A simple, cost-effective and miniaturized lab-on-a-chip platform has been developed amenable to perform simultaneous cultivation and detection of bacteria. A microfluidic chamber was integrated to screen-printed electrodes for electrochemical detection of bacteria. The temperature required for the bacterial culture was provided through the optimized laser-induced graphene heaters. The concentration of bacteria was quantified accurately with the three-electrode system in the range of 2 × 104 to 1.1 × 109 CFU/mL without any need of biological modifications to the electrodes. The viability of cultured bacteria in the microfluidic device was also confirmed through fluorescent imaging. Furthermore, the metabolic activity of the cultured bacteria was validated through a miniaturized microbial fuel cell. Furthermore, the specificity of electrodes was also performed through electrochemical technique. Finally, a handheld and portable lab-on-a-chip platform was realized by 3D packaging, integrated with a portable potentiostat for real-time and on-field applications.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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