A lab-on-chip platform for simultaneous culture and electrochemical detection of bacteria
Sangam Srikanth,
U.S. Jayapiriya,
Satish Kumar Dubey,
Arshad Javed,
Sanket Goel
Affiliations
Sangam Srikanth
Department of Mechanical Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India
U.S. Jayapiriya
Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India
Satish Kumar Dubey
Department of Mechanical Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India
Arshad Javed
Department of Mechanical Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India
Sanket Goel
Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; Corresponding author
Summary: A simple, cost-effective and miniaturized lab-on-a-chip platform has been developed amenable to perform simultaneous cultivation and detection of bacteria. A microfluidic chamber was integrated to screen-printed electrodes for electrochemical detection of bacteria. The temperature required for the bacterial culture was provided through the optimized laser-induced graphene heaters. The concentration of bacteria was quantified accurately with the three-electrode system in the range of 2 × 104 to 1.1 × 109 CFU/mL without any need of biological modifications to the electrodes. The viability of cultured bacteria in the microfluidic device was also confirmed through fluorescent imaging. Furthermore, the metabolic activity of the cultured bacteria was validated through a miniaturized microbial fuel cell. Furthermore, the specificity of electrodes was also performed through electrochemical technique. Finally, a handheld and portable lab-on-a-chip platform was realized by 3D packaging, integrated with a portable potentiostat for real-time and on-field applications.
