Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Hayato Fukusumi; Tomoko Shofuda; Yohei Bamba; Atsuyo Yamamoto; Daisuke Kanematsu; Yukako Handa; Keisuke Okita; Masaya Nakamura; Shinya Yamanaka; Hideyuki Okano; Yonehiro Kanemura

doi:10.1155/2016/7235757

Stem Cells International (Jan 2016)

Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Hayato Fukusumi,
Tomoko Shofuda,
Yohei Bamba,
Atsuyo Yamamoto,
Daisuke Kanematsu,
Yukako Handa,
Keisuke Okita,
Masaya Nakamura,
Shinya Yamanaka,
Hideyuki Okano,
Yonehiro Kanemura

Affiliations

Hayato Fukusumi: Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan
Tomoko Shofuda: Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan
Yohei Bamba: Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan
Atsuyo Yamamoto: Division of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan
Daisuke Kanematsu: Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan
Yukako Handa: Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan
Keisuke Okita: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan
Masaya Nakamura: Department of Orthopedic Surgery, Keio University School of Medicine, Tokyo 160-8582, Japan
Shinya Yamanaka: Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8507, Japan
Hideyuki Okano: Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan
Yonehiro Kanemura: Division of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan

DOI: https://doi.org/10.1155/2016/7235757
Journal volume & issue: Vol. 2016

Abstract

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Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

Published in Stem Cells International

ISSN: 1687-966X (Print); 1687-9678 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine
Website: https://onlinelibrary.wiley.com/journal/4162

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