The effect of perioperative analgesia with omnopon and parecoxib on the endocytic activity of murine phagocytes on the model of tumor surgery

Abstract

We used the model of surgical tumor removal to compare the effect of anesthesia with opioid analgesic omnopon and selective cyclooxygenase-2 inhibitor parecoxib on the endocytic activity of phagocytes of different localization sites. 50 C57/black mice were transplanted with Lewis lung carcinoma in the hind paw pad. After 22 days, the tumor paw was amputated. Analgesics (omnopon 10 mg/kg, parecoxib – 20 mg/kg) were administered 30 min before the operation and once per day for 3 days after the surgery. Assessment of the endocytic activity of phagocytes was performed by FACS analysis before the surgery, at days 1 and 3 after the surgery. It was found that parecoxib analgesia maintained the endocytic activity of blood and spleen phagocytes in the postoperative period. At day 3 after the surgery in parecoxib-treated animals phagocytic activity of splenic granulocytes were 2.2 times higher compared to that in the group receiving opioid analgesia. Phagocytic indices of monocytes in parecoxib-treated mice were also 1.6 and 2.5 times higher for blood and spleen monocytes, respectively. Thus, parecoxib analgesia maintained the activity of blood and spleen phagocytes in mice after the surgical tumor removal at a much higher level as compared with the omnopon analgesia.

Keywords

• phagocytic activity
• perioperative analgesia
• parecoxib
• opioid drugs