Frontiers in Plant Science (Nov 2022)

Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate

  • Na Han,
  • Wen-ji Geng,
  • Jian Li,
  • Shu-ting Liu,
  • Jie Zhang,
  • Yi-jie Wen,
  • Huai-hua Xu,
  • Meng-yuan Li,
  • Yan-ru Li,
  • Pei-pei Han

DOI
https://doi.org/10.3389/fpls.2022.1040596
Journal volume & issue
Vol. 13

Abstract

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Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level . Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 μM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plant-pathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out.

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