The applying of immunohistochemical method for evaluating the cytoprotective effect of antidiabetic drugs

Abstract

Resume. The article describes the experience of using the immunohistochemical method of analysis with anti-insulin monoclonal antibodies to assess the cytoprotective effect of lithium salts (carbonate and chloride) on pancreatic p-cells. The main advantage of this method over the classical staining methods, which reveal all types of Langerhans' cells, is selective insulin-producing p-cells identification. On the model of streptozotocin-induced type 2 diabetes in Wistar rats, a significant reduction in the p-cells number and a violation of their morphological structure was shown. Both lithium salts are known to be the GSK-3P inhibitors. Our experiments revealed the pronounced cytoprotective activity, consisting in restoring of the insulinocytes' morphological characteristics, increasing the p-cells absolute and relative quantity and the percentage of small and medium-sized islets. A satisfactory correlation between the degree of the lithium salts cytoprotective effect and their hypoglycemic activity has been revealed.

Keywords

• диабета 2 типа
• стрептозотоцин
• лития хлорид
• лития карбонат
• р-клетки
• цитопротекция
• diabetes 2 type
• streptozotocin
• gsk-3p
• lithium salts
• p-cells
• cytoprotection
• immunohistochemistry