Torvoside A extracted from Solanum torvum fruits does not directly affect 3T3-L1 –adipogenesis, adiponectin synthesis and glucose uptake

Abstract

The present study was aimed to determine the effects of Torvoside A from methanolic extract of Solanum torvum on adipogenesis, fat accumulation, and adiponectin synthesis as well as glucose uptake activities. For adipogenesis assay, 3T3-L1- preadipocytes were treated with Torvoside A (0, 5, 25, 50 µM) during 7 days of adipocyte differentiation, while 3T3-L1 mature adipocytes were treated with Torvoside A for 2 days in the fat accumulation study. There were no significant differences in the intracellular triglyceride among Torvoside A treated cells and the untreated control (p>0.05) in both assays. Torvoside A did not significantly change adiponectin synthesis in both transcriptional and translational levels (p>0.05), measured by real-time RTPCR and western blot, respectively. Finally, Torvoside A did not affect glucose uptake activity and GLUT4 transcript as well. These results can be concluded that Torvoside A could not directly promote adipocyte hyperplasia and hypertrophy as well as the anti-diabetic activities.

Keywords

• torvoside a
• adipogenesis
• glucose uptake
• solanum torvum
• fat accumulation