Effect of Transfer Temperature of Epididymis on Survival Rate of Semen and B6D2F1 Mice Developmental Capacity

Abstract

This study was conducted to evaluate the effect of transfer temperature of epididymis on survival rate of semen and development ability of B6D2F1 mice embryos. No significant differences were noted in the survival rate of semen (59.0% ± 0.1 vs. 47.6% ± 0.1), in vitro fertilization rate (90.7% ± 0.1 vs. 90.7% ± 0.1), developmental rate (90.0% ± 0.1 vs. 90.0% ± 0.1), and blastocysts formation rate (53.1% ± 0.2 vs. 52.3% ± 0.2) between groups. (NS; P>0.05). However, the zona hatched rate was significantly higher in the 4°C group compared to those of the 37°C group (47.8% ± 0.1 vs. 25.6% ± 0.2; p0.05). Taken altogether, it is expected to achieve the best developmental ability of B6D2F1 mice embryos in the transfer temperature of epididymis. Also these results can provide fundamental data to maximize culture condition for in vitro fertilization on B6D2F1 mice. In future, therefore, it is expected that results herein might be applied for in vitro culture of human embryos.

Keywords

• b6d2f1 mice
• epididymis
• in vitro fertilization
• inner cell mass
• oogenesis