Comparative transcriptome analysis provides a glance into the regulation of the Krüppel homolog 1 on the reproduction and diapause of the predatory ladybeetle, Coccinella septempunctata

Abstract

Abstract As a typical natural predator of agricultural pests, the lady beetle, Coccinella septempunctata, has been commercially mass-reared and widely employed in in pest management. The lady beetles can enter a long-term reproductive diapause phase in response to short photoperiods and low temperatures, for maintaining population during the overwintering process. Insect diapause is a physiological adaptative strategy that is characterized by a cessation of ovarian development, lipid accumulation and extended lifespan. Diapause regulating improve the long-term storage of C. septempunctata and release of natural enemies at the right time. In our previous research, as a main output of the juvenile hormone pathway, Krüppel homolog 1 (Kr-h1) was a key component of reproduction and diapause in C. septempunctata. However, the molecular mechanisms underlying the regulation of C. septempunctata reproduction and diapause by Kr-h1 transcription factor remains unknown. In this study, we utilized RNA-Sequencing to investigate the transcriptomic changes in C. septempunctata following RNA interference targeting the Kr-h1 gene. DEGs analysis revealed significant transcriptional alterations between the Kr-h1 knockdown group and the control group. Noteworthy findings include the downregulation of three genes related to reproduction (follicle cell protein,vitelline membrane protein, and vitellogenin) in the dsKr-h1 group, while genes involved in lipid metabolism, such as lipase and fatty acid synthase, were upregulated. These results suggested that Kr-h1 plays a critical role in the regulation of both reproductive processes and lipid metabolism in C. septempunctata. Our findings provided valuable insights into the molecular mechanisms regulating reproduction and diapause in C. septempunctata and contributed to the expanding understanding of the role of CsKr-h1 in insect physiology.

Keywords

• Coccinella septempunctata
• Comparative transcriptome
• Krüppel homolog 1
• Reproduction
• Diapause
• RNA interference