Effect of Amino Acids on the Production of Biosurfactant by Pediococcus Acidilactici F70

Abstract

In this research, the surface activity of bacterial supernatant and cell surface was measured by the method of oil drain ring. The influence of 19 kinds of amino acids (Histidine, Threonine, Valine, Isoleucine, Leucine, Phenylalanine, Arginine, Proline, Methionine, Tryptophan, Alanine, Glycine, Glutamine, Cysteine, Hydroxyproline, Valine, Asparagine, Proline, Glutamine, Serine, and Glutamic acid, Hydroxyproline, Tyrosine) on the production of biosurfactant by Pediococcus acidilactici F70 was studied by single factor experiment, and the main amino acids promoting the production of biosurfactant were selected by Plackett–Burman design. The results showed that the yield of biological surfactant with added amino acid increased, and the yield of Glutamine produced biosurfactant in the supernatant was the highest, which was two times higher than that of the control group (414.00mg/L); the yield of biosurfactant on the cell surface was the highest when Arginine was added, which was three times higher than that of the control group. In the end, 8 of the 19 amino acids (Glycine, Tryptophan, Proline, Methionine, Arginine, Leucine, Serine and Alanine) were selected to promote the production of biosurfactants of Pediococcus acidilactici F70. The results of Plackett–Burman design showed that Alanine, Proline and Leucine had significant effects on the production of biosurfactants.

Keywords

• pediococcus acidilactici f70
• biosurfactant
• amino acids
• plackett–burman design