A Python script to merge Sanger sequences

Cen Chen; Bingguo Lu; Xiaofang Huang; Chuyun Bi; Lili Zhao; Yunzhuo Hu; Xuanyang Chen; Shiqiang Lin; Kai Huang

doi:10.7717/peerj.11354

PeerJ (Apr 2021)

A Python script to merge Sanger sequences

Cen Chen,
Bingguo Lu,
Xiaofang Huang,
Chuyun Bi,
Lili Zhao,
Yunzhuo Hu,
Xuanyang Chen,
Shiqiang Lin,
Kai Huang

Affiliations

Cen Chen: Clinical Center for Human Genomic Research, Union Hospital, Huazhong University of Science and Technology, Wuhan, China
Bingguo Lu: Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Science, Fujian Normal University, Fuzhou, China
Xiaofang Huang: Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fujian Province Universities, Fuzhou, China
Chuyun Bi: Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fujian Province Universities, Fuzhou, China
Lili Zhao: State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control, Beijing, China
Yunzhuo Hu: Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fujian Province Universities, Fuzhou, China
Xuanyang Chen: Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fujian Province Universities, Fuzhou, China
Shiqiang Lin: Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fujian Province Universities, Fuzhou, China
Kai Huang: Clinical Center for Human Genomic Research, Union Hospital, Huazhong University of Science and Technology, Wuhan, China

DOI: https://doi.org/10.7717/peerj.11354
Journal volume & issue: Vol. 9
p. e11354

Abstract

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Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.

Published in PeerJ

ISSN: 2167-8359 (Online)
Publisher: PeerJ Inc.
Country of publisher: United States
LCC subjects: Medicine; Science: Biology (General)
Website: https://peerj.com/

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