Functional proteomic insights into the lateral oviduct-associated secretions of a bean bug
Ying Tian,
Hangpeng Xu,
Yanyan Yang,
Chuanxi Zhang,
Jianping Chen,
Junmin Li,
Jiabao Lu
Affiliations
Ying Tian
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
Hangpeng Xu
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
Yanyan Yang
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
Chuanxi Zhang
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China; Institute of Insect Science, Zhejiang University, Hangzhou 310058, China
Jianping Chen
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
Junmin Li
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
Jiabao Lu
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China; Corresponding author
Summary: The secretions within the lateral oviduct lumen of the insect female reproductive tract (FRT) play crucial roles in reproduction, yet their protein composition and functional mechanisms remain poorly understood. In this study, we revealed that the lateral oviduct of the bean bug, Riptortus pedestris, is composed of an outer muscle layer and an inner epithelium with a microvillar border. Proteomic analysis identified 159 FRT-highly expressed proteins, with functional characterization focusing on 10 putative secretory proteins, including 2 ovary-specific (RpLoap2 and RpLoap4), 7 oviduct-specific (RpLoap3 and RpLoap5-10), and 1 ovary-highly expressed (RpLoap1) genes. Notably, RNAi-mediated knockdown of RpLoap2 severely impaired choriogenesis, resulting in complete reproductive failure. These findings provide valuable insights into insect reproduction biology from the perspective of lateral oviduct–associated secretions and establish RpLoap2 as essential for successful reproduction in R. pedestris.
