Molecular and Clinical investigation of lumpy skin disease in cattle in AL .Qadissiyia province

Abstract

In the present study describes the clinical, histological, and molecular diagnosis of lumpy skin disease (LSD) which prevalence as Outbreak in AL -Qadissiyia province. Sixteen cases suspected infection with (LSD) in cattle depending on the clinical signes which appear clearly in diferent area in AL -Qadissiyia province. All suspected animals were clinically examined and a tentative diagnosis of LSD was done upon noticing of typical clinical signs . Specimens (skin biopsies) were collected from nodular skin lesions of infected animals for histopathological examination then the PCR assay was performed to confirm the found of disease in the suspected cases . The result of The physical and clinical examination revealed atypical clinical manifestation of (LSD) .histopathological result showed characteristic pathological change. The results of PCR (conventional PCR )technique for detection of specific primer P23 antigen gene (720bp)of( LSD)virus showed 14 out of 16 were positive result from collected samples . In conclusion the lumpy skin disease is wide spread in a AL -Qadissiyia province and the disease have a typical and characteristic clinical ,histopathological features that used in tentative diagnosis .PCR technique was rapid ,sensitive and specific for detection of the (LSD)virus and confirmative diagnosis ofdisease.

Keywords

• Cattle
• histopathology
• lumpy skin disease
• molecular
• PCR