Nature Communications (Apr 2022)

Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation

  • Mathieu Botte,
  • Dongchun Ni,
  • Stephan Schenck,
  • Iwan Zimmermann,
  • Mohamed Chami,
  • Nicolas Bocquet,
  • Pascal Egloff,
  • Denis Bucher,
  • Matilde Trabuco,
  • Robert K. Y. Cheng,
  • Janine D. Brunner,
  • Markus A. Seeger,
  • Henning Stahlberg,
  • Michael Hennig

DOI
https://doi.org/10.1038/s41467-022-29459-2
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 10

Abstract

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Lateral opening of the LptDE transporter in the outer membrane of Gram-negative bacteria is necessary for insertion of lipopolysaccharides. Here, Botte et al. report a cryo-EM structure of a partially opened LptDE transporter, in complex with rigid chaperones derived from nanobodies.