Strictosamide promotes wound healing through activation of the PI3K/AKT pathway
Gu-xu Ming,
Jun-yan Liu,
Yu-huang Wu,
Li-yan Li,
Xin-yue Ma,
Pei Liu,
Yi-peng Pan,
Xiao-ning He,
Yong-hui Li
Affiliations
Gu-xu Ming
Hainan Provincial Key Laboratory R&D on Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China
Jun-yan Liu
Hainan Provincial Key Laboratory R&D on Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China
Yu-huang Wu
Hainan Provincial Key Laboratory R&D on Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China
Li-yan Li
Hainan Provincial Key Laboratory R&D on Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China
Xin-yue Ma
Hainan Provincial Key Laboratory R&D on Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China
Pei Liu
The Second Affiliated Hospital, Hainan Medical University, Haikou, China
Yi-peng Pan
The Second Affiliated Hospital, Hainan Medical University, Haikou, China; Corresponding author.
Xiao-ning He
The Second Affiliated Hospital, Hainan Medical University, Haikou, China; Corresponding author.
Yong-hui Li
Hainan Provincial Key Laboratory R&D on Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China; The Second Affiliated Hospital, Hainan Medical University, Haikou, China; Corresponding author. Hainan Provincial Key Laboratory R&D on Tropical Herbs, Haikou Key Laboratory of Li Nationality Medicine, School of Pharmacy, Hainan Medical University, Haikou, China.
Nauclea officinalis, as a Chinese medicine in Hainan province, had the effect of treating lower limb ulcers, burn infections. In this paper, we studied the effect of Strictosamide (STR), the main bioactive compound in Nauclea officinals, on wound healing and explored its internal mechanism. Firstly, the wound healing potential of STR was evaluated in a rat model, demonstrating its ability to expedite wound healing, mitigate inflammatory infiltration, and enhance collagen deposition. Additionally, immunofluorescence analysis revealed that STR up-regulated the expression of CD31 and PCNA. Subsequently, target prediction, protein-protein interaction (PPI), gene ontology (GO), and pathway enrichment analyses were used to obtain potential targets, specific biological processes, and molecular mechanisms of STR for the potential treatment of wound healing. Furthermore, molecular docking was conducted to predict the binding affinity between STR and its associated targets. Additionally, in vivo and in vitro experiments confirmed that STR could increase the expression of P-PI3K, P-AKT and P-mTOR by activating the PI3K/AKT signaling pathway. In summary, this study provided a new explanation for the mechanism by which STR promotes wound healing through network pharmacology, suggesting that STR may be a new candidate for treating wound.
