Interactions Between 5-Fluorouracil and PAMAM Dendrimers Studies by NMR Spectroscopy

Abstract

Interactions between 5-fluorouracil (5-FU) and PAMAM in D2O solution were studied by NMR spectroscopy. The results of 1H NMR chemical shift titration experiments demonstrated that 5-FU molecules bonded to the surface of PAMAM, and the results of 13C NMR chemical shift titrations experiments disclosed that hydrogen-bond interactions and/or hydrophobic interaction ins existed in the interior pockets of PAMAM and 5-FU. These results were further supported by 1H and 19F relaxation time measurements. The NOESY experiment was used to confirm the presence of 5-FU encapsulation in the PAMAM-5-FU complexes. This study provides a methodology for studying the interactions between dendrimers and drug molecules.

Keywords

• liquid-state nmr
• interaction
• pamam dendrimers
• 5-fluorouracil