Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA

Anustup Poddar; Muhammad S. Azam; Tunc Kayikcioglu; Maksym Bobrovskyy; Jichuan Zhang; Xiangqian Ma; Piyush Labhsetwar; Jingyi Fei; Digvijay Singh; Zaida Luthey-Schulten; Carin K. Vanderpool; Taekjip Ha

doi:10.1038/s41467-021-21144-0

Nature Communications (Feb 2021)

Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA

Anustup Poddar,
Muhammad S. Azam,
Tunc Kayikcioglu,
Maksym Bobrovskyy,
Jichuan Zhang,
Xiangqian Ma,
Piyush Labhsetwar,
Jingyi Fei,
Digvijay Singh,
Zaida Luthey-Schulten,
Carin K. Vanderpool,
Taekjip Ha

Affiliations

Anustup Poddar: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
Muhammad S. Azam: Department of Microbiology, University of Illinois at Urbana-Champaign
Tunc Kayikcioglu: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
Maksym Bobrovskyy: Department of Microbiology, University of Illinois at Urbana-Champaign
Jichuan Zhang: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
Xiangqian Ma: Department of Microbiology, University of Illinois at Urbana-Champaign
Piyush Labhsetwar: Department of Chemistry, University of Illinois at Urbana-Champaign
Jingyi Fei: Department of Biochemistry and Molecular Biology, University of Chicago
Digvijay Singh: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
Zaida Luthey-Schulten: Department of Chemistry, University of Illinois at Urbana-Champaign
Carin K. Vanderpool: Department of Microbiology, University of Illinois at Urbana-Champaign
Taekjip Ha: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine

DOI: https://doi.org/10.1038/s41467-021-21144-0
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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Bacterial small RNA SgrS binds and regulates its primary target, ptsG mRNA. Here the authors employ Sort-Seq and super resolution imaging to investigate in vivo target recognition and rejection kinetics of SgrS.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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