The complete chloroplast genome and characteristics analysis of Lonicera harae (Caprifoliaceae)

Abstract

Herein, we determined and analyzed the chloroplast (cp) genome sequence of Lonicera harae for the first time. The chloroplast genome was 154,959 bp and contained a large single copy (LSC) of approximately 88,849 bp as well as a small single copy (SSC) of 18,832 bp separated by a pair of 23,639 bp inverted repeats (IRs). The overall G+C content in the L. harae chloroplast genome was 38.4%. A total of 114 unique genes were annotated, comprising 80 protein-coding genes (PCGs), 30 transfer RNAs (tRNAs), and four ribosomal RNAs (rRNAs). Of these, 17 contained one or two introns. Phylogenetic analysis of 54 accessions revealed that L. harae forms a clade with L. fragrantissima. This newly sequenced chloroplast genome provides valuable molecular phylogenomic information for future studies to determine the taxonomic position of L. harae and the phylogenetic relationships within the genus Lonicera.

Keywords

• Caprifoliaceae
• chloroplast genome
• Lonicera harae
• phylogeny