Possible Spreading of SARS-CoV-2 from Humans to Captive Non-Human Primates in the Peruvian Amazon
Andrea Tavera Gonzales,
Jhonathan Bazalar Gonzales,
Thalía Silvestre Espejo,
Milagros Leiva Galarza,
Carmen Rodríguez Cueva,
Dennis Carhuaricra Huamán,
Luis Luna Espinoza,
Abelardo Maturrano Hernández
Affiliations
Andrea Tavera Gonzales
Research Group in Biotechnology Applied to Animal Health, Production and Conservation (SANIGEN), Laboratorio de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru
Jhonathan Bazalar Gonzales
Research Group in Biotechnology Applied to Animal Health, Production and Conservation (SANIGEN), Laboratorio de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru
Thalía Silvestre Espejo
Research Group in Biotechnology Applied to Animal Health, Production and Conservation (SANIGEN), Laboratorio de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru
Milagros Leiva Galarza
Research Group in Biotechnology Applied to Animal Health, Production and Conservation (SANIGEN), Laboratorio de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru
Carmen Rodríguez Cueva
Research Group in Biotechnology Applied to Animal Health, Production and Conservation (SANIGEN), Laboratorio de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru
Dennis Carhuaricra Huamán
Research Group in Biotechnology Applied to Animal Health, Production and Conservation (SANIGEN), Laboratorio de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru
Luis Luna Espinoza
Research Group in Biotechnology Applied to Animal Health, Production and Conservation (SANIGEN), Laboratorio de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru
Abelardo Maturrano Hernández
Research Group in Biotechnology Applied to Animal Health, Production and Conservation (SANIGEN), Laboratorio de Biología y Genética Molecular, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru
Human-to-animal transmission events of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) have been reported in both domestic and wild species worldwide. Despite the high rates of contagion and mortality during the COVID-19 (Coronavirus Diseases 2019) pandemic in Peru, no instances of natural virus infection have been documented in wild animals, particularly in the Amazonian regions where human–wildlife interactions are prevalent. In this study, we conducted a surveillance investigation using viral RNA sequencing of fecal samples collected from 76 captive and semi-captive non-human primates (NHPs) in the Loreto, Ucayali, and Madre de Dios regions between August 2022 and February 2023. We detected a segment of the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2 by metagenomic sequencing in a pooled fecal sample from captive white-fronted capuchins (Cebus unicolor) at a rescue center in Bello Horizonte, Ucayali. Phylogenetic analysis further confirmed that the retrieved partial sequence of the RdRp gene matched the SARS-CoV-2 genome. This study represents the first documented instance of molecular SARS-CoV-2 detection in NHPs in the Peruvian Amazon, underscoring the adverse impact of anthropic activities on the human–NHP interface and emphasizing the importance of ongoing surveillance for early detection and prediction of future emergence of new SARS-CoV-2 variants in animals.
