Scientia Agraria Paranaensis (Sep 2015)
DISPONIBILIDADE DE LUZ, TIPO DE VEDAÇÃO E DE FRASCO NA GERMINAÇÃO E CRESCIMENTO INICIAL IN VITRO DE PLÂNTULAS DE CUBIU (Solanum sessiliflorum DUNAL)
Abstract
This work aimed to evaluate the different types of sealing and flask size in relation to the rates of germination and biomass partitioning in seedlings of two varieties of Solanum sessiliflorum Dunal. Seeds of varieties Santa Luzia and Thais were inoculated into flasks of varying sizes, containing culture medium Murashige and Skoog (1962) with 50% of the concentration and sealed with different materials. It was found shriveling leaf in plantlets of both varieties grown in sealed flasks with polyvinyl chloride (PVC). The varieties Santa Luzia and Thais showed high germination percentage in flasks sealed with PVC and aluminum foil. Furthermore, the type of flask and sealing influenced the accumulation of assimilates in both varieties. It was found yet, the highest average of fresh matter in large flasks and sealed with aluminum for the Thais variety, while the Santa Luzia variety, the flask size does not interfere with weight, but those sealed with aluminum have a higher average weight. The dry matter accumulation for Thais variety were higher for plantlets cultivated in large flasks sealed with PVC, and for Santa Luzia variety were large flasks or sealed with PVC. Both varieties, when grown with aluminum foil have the highest average length of the aerial part. Plantlets cultivated in small flasks and sealed with PVC presented higher rootlength. It was concluded that the leaf shriveling was remarkable in flasks sealed with PVC and the accumulation of assimilates is influenced by the type of flask and seal for both varieties in the presence of light.
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