Agronomy (Dec 2022)

Evaluation of Indian Mustard Genotypes for White Rust Resistance Using <i>BjuWRR1</i>Gene and Their Phenotypic Performance

  • Yengkhom Sanatombi Devi,
  • Th. Renuka Devi,
  • Ajay Kumar Thakur,
  • Umakanta Ngangkham,
  • H. Nanita Devi,
  • Pramesh Kh.,
  • Bireswar Sinha,
  • Pushparani Senjam,
  • N. Brajendra Singh,
  • Lokesh Kumar Mishra

DOI
https://doi.org/10.3390/agronomy12123122
Journal volume & issue
Vol. 12, no. 12
p. 3122

Abstract

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The present investigation was carried out to identify the potential donors of resistant gene(s)/the source of white rust disease in B. juncea using 30 genotypes, including locally adapted accessions and advanced breeding lines. Out of 30 genotypes, ten lines viz. Bio-YSR, CAULC-1, CAULC-2, CAULC-3, CAULC-4, CAURM-2, CAULR-7, CAURM-4, CAURM 4-1, and CAURM 4-2 exhibited a lower PDI value (lesser than mean 10.83) with a superior agronomic performance related with the disease. The evaluation of these ten genotypes for the presence of the BjuWRR1 gene using a gene-based marker depicted the presence of the functional allele of the BjuWRR1 gene in the five genotypes viz., Bio-YSR, CAULC-1, CAULC-3, CAURM 4-1 and CAURM 4-2. When compared with the sequenced amplicon of these genotypes, it is found to be identical with that of an east European Brassica juncea line, Donskaja-IV, the completely resistant genotype against various isolates of Albugo candida. The findings from the present study suggested that besides Bio-YSR, the local lines of Manipur CAULC-1 (Local Yella of Potshangbam) and CAULC-3 (Local Yella of Kakching Lamjao) can be used as the potential white rust resistance sources/donors in disease resistance breeding programmes for the development of elite B. juncea cultivars in the future. In addition to the local lines, two improved advanced lines, viz. CAURM 4-1 and CAURM 4-2, obtained from a hybridization programme, may be further evaluated for releasing resistant varieties against white rust.

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