Evaluation of Indian Mustard Genotypes for White Rust Resistance Using <i>BjuWRR1</i>Gene and Their Phenotypic Performance
Yengkhom Sanatombi Devi,
Th. Renuka Devi,
Ajay Kumar Thakur,
Umakanta Ngangkham,
H. Nanita Devi,
Pramesh Kh.,
Bireswar Sinha,
Pushparani Senjam,
N. Brajendra Singh,
Lokesh Kumar Mishra
Affiliations
Yengkhom Sanatombi Devi
Department of Genetics and Plant Breeding, College of Agriculture, Central Agricultural University, Imphal 795004, Manipur, India
Th. Renuka Devi
Department of Genetics and Plant Breeding, College of Agriculture, Central Agricultural University, Imphal 795004, Manipur, India
Ajay Kumar Thakur
Directorate of Rapeseed-Mustard Research (ICAR), Bharatpur 321303, Rajasthan, India
Umakanta Ngangkham
ICAR Research Complex for NEH Region, Manipur Centre, Imphal 795004, Manipur, India
H. Nanita Devi
All India Co-ordinated Research Project (Soybean), College of Agriculture, Central Agricultural University, Imphal 795004, Manipur, India
Pramesh Kh.
All India Co-ordinated Research Project (Groundnut), College of Agriculture, Central Agricultural University, Imphal 795004, Manipur, India
Bireswar Sinha
Department of Plant Pathology, College of Agriculture, Central Agricultural University, Imphal 795004, Manipur, India
Pushparani Senjam
All India Co-ordinated Research Project-Rapeseed-Mustard, College of Agriculture, Central Agricultural University, Imphal 795004, Manipur, India
N. Brajendra Singh
Department of Genetics and Plant Breeding, College of Agriculture, Central Agricultural University, Imphal 795004, Manipur, India
Lokesh Kumar Mishra
Department of Biochemistry, Physiology, Microbiology and Environmental Science, College of Agriculture, Central Agricultural University, Imphal 795004, Manipur, India
The present investigation was carried out to identify the potential donors of resistant gene(s)/the source of white rust disease in B. juncea using 30 genotypes, including locally adapted accessions and advanced breeding lines. Out of 30 genotypes, ten lines viz. Bio-YSR, CAULC-1, CAULC-2, CAULC-3, CAULC-4, CAURM-2, CAULR-7, CAURM-4, CAURM 4-1, and CAURM 4-2 exhibited a lower PDI value (lesser than mean 10.83) with a superior agronomic performance related with the disease. The evaluation of these ten genotypes for the presence of the BjuWRR1 gene using a gene-based marker depicted the presence of the functional allele of the BjuWRR1 gene in the five genotypes viz., Bio-YSR, CAULC-1, CAULC-3, CAURM 4-1 and CAURM 4-2. When compared with the sequenced amplicon of these genotypes, it is found to be identical with that of an east European Brassica juncea line, Donskaja-IV, the completely resistant genotype against various isolates of Albugo candida. The findings from the present study suggested that besides Bio-YSR, the local lines of Manipur CAULC-1 (Local Yella of Potshangbam) and CAULC-3 (Local Yella of Kakching Lamjao) can be used as the potential white rust resistance sources/donors in disease resistance breeding programmes for the development of elite B. juncea cultivars in the future. In addition to the local lines, two improved advanced lines, viz. CAURM 4-1 and CAURM 4-2, obtained from a hybridization programme, may be further evaluated for releasing resistant varieties against white rust.
