Prognostic Role of RASSF1A, SOX17 and Wif-1 Promoter Methylation Status in Cell-Free DNA of Advanced Gastric Cancer Patients

Evangelos I. Karamitrousis BSc, MSc, MD, PhD; Ioanna Balgkouranidou BSc, PhD; Nikolaos Xenidis MD, PhD; Kyriakos Amarantidis MD, PhD; Eirini Biziota MD, PhD; Triantafyllia Koukaki MD; Grigorios Trypsianis BSc, PhD; Anastasios Karayiannakis MSc, MD, PhD; Helen Bolanaki MD; George Kolios MD, PhD; Evi Lianidou BSc, MSc, PhD; Stylianos Kakolyris MD, PhD

doi:10.1177/1533033820973279

Technology in Cancer Research & Treatment (Apr 2021)

Prognostic Role of RASSF1A, SOX17 and Wif-1 Promoter Methylation Status in Cell-Free DNA of Advanced Gastric Cancer Patients

Evangelos I. Karamitrousis BSc, MSc, MD, PhD,
Ioanna Balgkouranidou BSc, PhD,
Nikolaos Xenidis MD, PhD,
Kyriakos Amarantidis MD, PhD,
Eirini Biziota MD, PhD,
Triantafyllia Koukaki MD,
Grigorios Trypsianis BSc, PhD,
Anastasios Karayiannakis MSc, MD, PhD,
Helen Bolanaki MD,
George Kolios MD, PhD,
Evi Lianidou BSc, MSc, PhD,
Stylianos Kakolyris MD, PhD

Affiliations

Evangelos I. Karamitrousis BSc, MSc, MD, PhD: Department of Oncology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Ioanna Balgkouranidou BSc, PhD: Department of Oncology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Nikolaos Xenidis MD, PhD: Department of Oncology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Kyriakos Amarantidis MD, PhD: Department of Oncology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Eirini Biziota MD, PhD: Department of Oncology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Triantafyllia Koukaki MD: Department of Oncology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Grigorios Trypsianis BSc, PhD: Department of Medical Statistics, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Anastasios Karayiannakis MSc, MD, PhD: Second Department of Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Helen Bolanaki MD: Second Department of Surgery, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
George Kolios MD, PhD: Laboratory of Pharmacology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece
Evi Lianidou BSc, MSc, PhD: Department of Chemistry, Analysis of Circulating Tumor Cells, Lab of Analytical Chemistry, University of Athens, Athens, Greece
Stylianos Kakolyris MD, PhD: Department of Oncology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece

DOI: https://doi.org/10.1177/1533033820973279
Journal volume & issue: Vol. 20

Abstract

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Epigenetic modification of several genes is a key component in the development of gastric cancer. The methylation status of RASSF1A , SOX17 and Wif-1 genes was evaluated in the cell free circulating DNA of 70 patients with advanced gastric cancer, using methylation-specific PCR. Patients with higher cell-free DNA concentration seem to have lower PFS, than patients with lower cell-free DNA concentration (p = 0.001). RASSF1A was the tumor suppressor gene, most frequently methylated in metastatic gastric cancer patients, followed by SOX17 and Wif-1 (74.3%, 60.0% and 47.1%, respectively). Patients having the SOX17 promoter methylated, had lower progression free survival and overall survival, than unmethylated ones (p < 0.001). Patients having the Wif-1 promoter methylated, had lower progression free survival and overall survival, than unmethylated ones (p = 0.001). Patients having the RASSF1A promoter methylated, had lower progression free survival and overall survival, than unmethylated ones (p = 0.004). Promoter methylation of the examined genes was significantly associated with a decrease in progression free survival and overall survival, comparing to that of patients without methylation. Simultaneous methylation of the above genes was associated with even worse progression free survival and overall survival. The methylation of RASSF1A , SOX-17 and Wif-1 and genes, is a frequent epigenetic event in patients with advanced gastric cancer.

Published in Technology in Cancer Research & Treatment

ISSN: 1533-0346 (Print); 1533-0338 (Online)
Publisher: SAGE Publishing
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://journals.sagepub.com/home/tct

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