Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout

Abstract

Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene of this protein. This will allow, at no additional cost, to increase its safety for children who are breast-fed. In this article, we report on the development of a system for gene editing to create cows with a knockout of the beta-lactoglobulin gene.