Chloroplast genome and nuclear loci data for 71 Medicago species

Abstract

We present a dataset containing nuclear and chloroplast sequences for 71 species in genus Medicago (Fabaceae), as well as for 8 species in genera Melilotus and Trigonella. Sequence data for a total of 130 samples was obtained with high-throughput sequencing of enriched genomic DNA libraries targeting 61 single-copy nuclear genes from across the Medicago truncatula genome. Chloroplast sequence reads were also generated, allowing for the recovery of chloroplast genome sequences for all 130 samples. A fully-resolved phylogenetic tree was inferred from the chloroplast dataset using maximum-likelihoood methods. More than 80% of accepted Medicago species are represented in this dataset, including three subspecies of Medicago sativa (alfalfa). These data can be further utilised for phylogenetic analyses in Medicago and related genera, but also for probe and primer design and plant breeding studies.

Keywords

• Sequence-capture
• Phylogenetics
• Genomics
• Fabaceae