Salvianolic acid B alleviates LPS-induced acute lung injury in mice

Abstract

Objective To study whether salvianolic acid B(SAB) can reduce lipopolysaccharide(LPS)-induced acute lung injury. Methods Fourty-eight mice were randomly divided into control group, model group (intratracheal instillation of LPS), SAB low/medium/high dose intervention group, and sulforaphane positive control group. The lung wet / dry weight ratio (W/D) was recorded; The protein concentration of alveolar lavage fluid (BALF) was detected; Lung tissue pathological damage was evaluated by HE staining and microoscopy; TNF-α,IL-1β and IL-6 in BALF were detected by ELISA.Western blot was used to detect the protein expression of Nrf2, NQO1, HO1, Keap1,NF-κB and p-NF-κB in lung tissue. Results Compared with the control group, pathological changes were observed in the lung tissue of the model group, W/D, protein concentration in BALF and inflammatory cytokine content were significantly increased (P＜0.05). Compared with the model group, SAB intervention group had significant changes (P＜0.05) that alleviated the oxidative stress induced by LPS, increased the expression of Keap1(P＜0.01), increased the expression of Nrf2, NQO1 and HO1 (P＜0.01), and inhibited the activation of NF-κB and p-NF-κB induced by LPS. Conclusions SAB can alleviate LPS-induced acute lung injury of mice.

Keywords

• salvianolic acid b|acute lung injury|oxidative stress