精准医学杂志 (Oct 2023)
RELATIONSHIP BETWEEN HELICOBACTER PYLORI-ASSOCIATED GASTRITIS AND GASTRIC MUCOSAL OCCLUDIN IN CHILDREN
Abstract
Objective To explore the relationship between Helicobacter pylori (Hp) type, positive expression rate of gastric mucosal occludin and degree of gastric mucosal inflammation in children with Hp-associated gastritis. Methods Ninety children who received gastroscopy and were diagnosed with Hp-associated gastritis in our hospital from June 2018 to December 2021 were selected as the research object. The expression of serum Hp antibody in children was measured by Western blot. The strains of Hp were serotyped. The pathological changes of gastric mucosa in children were observed. The positive expression rate of gastric mucosal occludin was determined by quantitative immunohistochemistry method. The relationship between Hp strain type, positive expression rate of gastric mucosal occludin and degree of gastric mucosal inflammation was analyzed. Results The positive expression rate of gastric mucosal occludin in children infected with type Ⅰ strain was significantly lower than that in children infected with type Ⅱ strain or intermediate type strain (P<0.05), but there was no significant difference in the positive expression rate between children infected with type Ⅱ strain and those infected with intermediate type strain (P>0.05). There were significant differences in the degree of gastric mucosal inflammation among children infected with different Hp strains (H=18.013,P<0.01); the degree was significantly less severe in children infected with type Ⅱ strain than in children infected with typeⅠstrain or intermediate type strain (P<0.05), but there was no significant difference in the degree between children infected with typeⅠstrain and those infected with intermediate type strain (P>0.05). The positive expression rates of occludin in children with mild, moderate, and severe gastric mucosal inflammation were 80.0%, 35.1%, and 11.6%, respectively (χ2=19.655,P<0.05). Conclusion The positive expression rate of occludin in the gastric mucosa of children with Hp-associated gastritis is related to the type of Hp strain and the degree of gastric mucosal inflammation, and the positive expression rate of occludin is the lowest when typeⅠstrain is infected and gastric mucosal inflammation is severe. The degree of gastric mucosal inflammation caused by different types of strain infection is different, and the degree of gastric mucosal inflammation caused by type Ⅱ strain infection is the mildest. Hp antibody typing detection may predict the tight junction and inflammation degree of gastric mucosa, and occludin is expected to become an important target for the treatment of Hp-related gastritis.
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