Wavefront correction with image-based interferometric focus sensing in two-photon microscopy
Yang Ruiwen,
Yang Yanlong,
Wu Tengfei,
Zhang Yang,
Dan Dan,
Min Junwei,
Yu Xianghua,
Dai Taiqiang,
Kong Liang,
Li Li,
Yao Baoli
Affiliations
Yang Ruiwen
State Key Laboratory of Ultrafast Optical Science and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an710119, China
Yang Yanlong
State Key Laboratory of Ultrafast Optical Science and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an710119, China
Wu Tengfei
State Key Laboratory of Ultrafast Optical Science and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an710119, China
Zhang Yang
State Key Laboratory of Ultrafast Optical Science and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an710119, China
Dan Dan
State Key Laboratory of Ultrafast Optical Science and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an710119, China
Min Junwei
State Key Laboratory of Ultrafast Optical Science and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an710119, China
Yu Xianghua
State Key Laboratory of Ultrafast Optical Science and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an710119, China
Dai Taiqiang
Department of Oral and Maxillofacial Surgery, State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, School of Stomatology, The Fourth Military Medical University, Xi’an710032, China
Kong Liang
Department of Oral and Maxillofacial Surgery, State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, School of Stomatology, The Fourth Military Medical University, Xi’an710032, China
Li Li
Department of Ophthalmology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an710061, China
Yao Baoli
State Key Laboratory of Ultrafast Optical Science and Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an710119, China
Adaptive optics is a technology that corrects wavefront distortions to enhance image quality. Interferometric focus sensing (IFS), a relatively recently proposed method within the field of adaptive optics, has demonstrated effectiveness in correcting complex aberrations in deep tissue imaging. This approach determines the correction pattern based on a single location within the sample. In this paper, we propose an image-based interferometric focus sensing (IBIFS) method in a conjugate adaptive optics configuration that progressively estimates and corrects the wavefront over the entire field of view by monitoring the feedback of image quality metrics. The sample conjugate configuration allows for the correction of multiple points across the full field of view by sequentially measuring the correction pattern for each point. We experimentally demonstrate our method on both the fluorescent beads and the mouse brain slices using a custom-built two-photon microscope. We show that our approach has a large effective field of view as well as more stable optimization results compared to the region of interest based method.
