Co-Functionalization of Gold Nanoparticles with C7H2 and HuAL1 Peptides: Enhanced Antimicrobial and Antitumoral Activities
Daniela M. D. Formaggio,
Jéssica A. Magalhães,
Vitor M. Andrade,
Katia Conceição,
Juliana M. Anastácio,
Gabrielli S. Santiago,
Denise C. Arruda,
Dayane B. Tada
Affiliations
Daniela M. D. Formaggio
Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, SP, Brazil
Jéssica A. Magalhães
Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, SP, Brazil
Vitor M. Andrade
Laboratory of Peptide Biochemistry, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, SP, Brazil
Katia Conceição
Laboratory of Peptide Biochemistry, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, SP, Brazil
Juliana M. Anastácio
Laboratory of Experimental Cancer Biology, University of Mogi das Cruzes (UMC), Mogi das Cruzes 08780-911, SP, Brazil
Gabrielli S. Santiago
Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, SP, Brazil
Denise C. Arruda
Laboratory of Experimental Cancer Biology, University of Mogi das Cruzes (UMC), Mogi das Cruzes 08780-911, SP, Brazil
Dayane B. Tada
Laboratory of Nanomaterials and Nanotoxicology, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, SP, Brazil
The functionalization of nanoparticles with therapeutic peptides has been pointed out as a promising strategy to improve the applications of these molecules in the field of health sciences. Peptides are highly bioactive but face several limitations such as low bioavailability due to the difficulty of overcoming the physiological barriers in the body and their degradation by enzymes. In this work, gold nanoparticles (AuNPs) were co-functionalized with two therapeutic peptides simultaneously. The peptides from the complementary determining region of monoclonal antibodies, composed of the amino acid sequences YISCYNGATSYNQKFK (C7H2) and RASQSVSSYLA (HuAL1) were chosen for having exhibited antitumor and antimicrobial activity before. The peptides-conjugated AuNPs were characterized regarding size, morphology, and metal concentration by using TEM, dynamic light scattering, and ICP-OES techniques. Then, peptides-conjugated AuNPs were evaluated regarding the antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. The antitumoral activity was evaluated in vitro by cell viability assays with metastatic melanoma cell line (B16F10-Nex2) and the cytotoxicity was evaluated against human foreskin fibroblast (Hs68) cell line. Finally, in vivo assays were performed by using a syngeneic animal model of metastatic melanoma. Our findings have highlighted the potential application of the dual-peptide AuNPs in order to enhance the antitumor and antimicrobial activity of peptides.
