Nature Communications (Jan 2016)

The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs shows ligand-dependent accessibility bursts

  • Arlie J. Rinaldi,
  • Paul E. Lund,
  • Mario R. Blanco,
  • Nils G. Walter

DOI
https://doi.org/10.1038/ncomms9976
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 10

Abstract

Read online

In response to intracellular signals, bacterial translational riboswitches embedded in mRNAs can regulate gene expression through inhibition of translation initiation. Here, the authors describe SiM-KARTS, a novel approach for detecting changes in the structure of single RNA molecules in response to a ligand.