Evaluation of stem cell differentiation medicated with calcium phosphate nanoparticles in chlorohexidine paste

Abstract

Abstract Background Our present study aimed to evaluate the effect of calcium phosphate nanoparticles in chlorohexidine paste on the odontogenic differentiation of human dental pulp stem cells. The human dental pulp stem cells (HDPSCs) were examined for viability and proliferation via trypan blue stain test and MTT assay. This was followed by evaluating the alkaline phosphatase assay and detecting the Dentin matrix protein (DMP-1) marker via immunofluorescence staining to confirm the odontogenic differentiation. Results In viability testing, nano calcium phosphate in Chlorohexidine (CHX) and nano calcium hydroxide showed no significant difference regarding trypan blue staining and MTT assay. Moreover, DMP-1 marker detection revealed a higher expression with nano calcium hydroxide compared to nano calcium phosphate in CHX, while ALP showed no significant difference. Conclusion Based on the analysis conveyed, it can be concluded that nano calcium phosphate in CHX can induce the proliferation and differentiation of HDPSCs.

Keywords

• HDPSCs
• Calcium phosphate
• DMP-1
• ALP