On the interaction and nanoplasmonics of gold nanoparticles and lipoproteins
Andrea Zendrini,
Jacopo Cardellini,
Roberto Frigerio,
Marianna Bertoni,
Debora Berti,
Paolo Bergese
Affiliations
Andrea Zendrini
Department of Molecular and Translational Medicine, Università degli Studi di Brescia, Viale Europa 11, 25123, Brescia, Italy; Center for Colloid and Surface Science (CSGI), Via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy; Corresponding author. Department of Molecular and Translational Medicine, Università degli Studi di Brescia, Viale Europa 11, 25123, Brescia, Italy.
Jacopo Cardellini
Center for Colloid and Surface Science (CSGI), Via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy; Department of Chemistry “Ugo Schiff”, Università degli Studi di Firenze, Via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy
Roberto Frigerio
Department of Molecular and Translational Medicine, Università degli Studi di Brescia, Viale Europa 11, 25123, Brescia, Italy; Istituto di Scienze e Tecnologie Chimiche “Giulio Natta”─National Research Council of Italy (SCITEC-CNR), 20131, Milano, Italy
Marianna Bertoni
Department of Molecular and Translational Medicine, Università degli Studi di Brescia, Viale Europa 11, 25123, Brescia, Italy
Debora Berti
Center for Colloid and Surface Science (CSGI), Via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy; Department of Chemistry “Ugo Schiff”, Università degli Studi di Firenze, Via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy
Paolo Bergese
Department of Molecular and Translational Medicine, Università degli Studi di Brescia, Viale Europa 11, 25123, Brescia, Italy; Center for Colloid and Surface Science (CSGI), Via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy; National Inter-university Consortium of Materials Science and Technology (INSTM), Via Giuseppe Giusti 9, 50121, Firenze, Italy; Corresponding author. Department of Molecular and Translational Medicine, Università degli Studi di Brescia, Viale Europa 11, 25123, Brescia, Italy.
The extracellular space is nanostructured, populated by heterogeneous classes of nanoparticles, e.g., extracellular vesicles and lipoproteins, which “made by cells for cells'' mediate intercellular, inter-organ, cross-species, and cross-kingdom communication. However, while techniques to study ENP biology in-vitro and in-vivo are becoming available, knowledge of their colloidal and interfacial properties is poor, although much needed. This paper experimentally shows, for the first time, that the aggregation of citrate-capped gold nanoparticles (AuNPs) triggered by lipid vesicle membranes and the related characteristic redshift of the plasmonic signature also applies/extends to lipoproteins. Such interaction leads to the formation of AuNP-lipoprotein hybrid nanostructures and is sensitive to lipoprotein classes and AuNP/lipoprotein molar ratio, paving the way to further synthetic and analytical developments.
