Single cell transcriptomics of the cerebral cortex of mice lacking the PRC2 gene eed

Abstract

Abstract Objective The Polycomb Repressive Complex 2 (PRC2) regulates neural stem cell behaviour during development of the cerebral cortex, yet how the loss of PRC2 developmentally influences cell identity in the mature brain is poorly defined. Using a mouse model in which the PRC2 gene Embryonic ectoderm development (Eed) was conditionally deleted from the developing mouse dorsal telencephalon, we performed single nuclei RNA sequencing (snRNA-seq) on the cortical plate of an adult heterozygote Eed knockout mouse and an adult homozygote Eed knockout mouse compared to a littermate control. This work was part of a larger effort to understand consequences of mutations to PRC2 within the mature brain. Results Here we provide snRNA-seq data from the cortical plate of an adult heterozygous conditional Eed knockout, an adult homozygous conditional Eed knockout and an adult control mouse. This data provides insight on how loss of PRC2 function during development affects cell identity in the mature cortex.

Keywords

• Single nuclei RNA-sequencing (snRNA-seq)
• Eed
• Polycomb repressive complex 2 (PRC2)
• Cerebral cortex