Oleic acid as a restorative agent in alleviating adrenaline induced altered morphofunctional milieu of gastric tissue and mitochondria
Sanatan Mishra,
Aindrila Chattopadhyay,
Shamreen Naaz,
Adrita Banerjee,
Arnab Kumar Ghosh,
Palash Kumar Pal,
Tuhin Bhattacharya,
Ankur Das,
Sreya Chattopadhyay,
Debasish Bandyopadhyay
Affiliations
Sanatan Mishra
Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata, 700009, India; Department of Physiology, Vidyasagar College, 39, Sankar Ghosh Lane, Kolkata, 700006, India
Aindrila Chattopadhyay
Department of Physiology, Vidyasagar College, 39, Sankar Ghosh Lane, Kolkata, 700006, India
Shamreen Naaz
Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata, 700009, India; Department of Physiology, Vidyasagar College for Women, 39, Sankar Ghosh Lane, Kolkata, 700006, India
Adrita Banerjee
Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata, 700009, India; Department of Physiology, Vidyasagar College, 39, Sankar Ghosh Lane, Kolkata, 700006, India
Arnab Kumar Ghosh
Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata, 700009, India
Palash Kumar Pal
Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata, 700009, India
Tuhin Bhattacharya
Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata, 700009, India
Ankur Das
Department of Physiology, University of Calcutta, Rajabazar Science College Campus, 92, APC Road, Kolkata 700 009, India
Sreya Chattopadhyay
Department of Physiology, University of Calcutta, Rajabazar Science College Campus, 92, APC Road, Kolkata 700 009, India
Debasish Bandyopadhyay
Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, 92, A.P.C. Road, Kolkata, 700009, India; Corresponding author.
The role of oleic acid as a protective antioxidant has recently been recognized. The present study is aimed to explore whether oleic acid can afford protection to rat gastric tissue when challenged with adrenaline. Sixty adult healthy male albino rats were divided into 10 groups comprising of 6 animals each. First group constituted the control. Rats of the second group were injected sub-cutaneously with adrenaline bitartrate at the dose of 0.3mg/kg body weight, every day for a period of 17 days. Rats of the third, to the sixth groups were orally fed with different doses of oleic acid (2.5, 5, 10, 20 mg/kg body weight/day) respectively. The rats of seventh to tenth groups were orally fed with doses of oleic acid as mentioned above and subsequently injected with adrenaline bitartrate at 0.3mg/kg body weight sub-cutaneously. After the treatment period, the animals were euthanized through cervical dislocation following light ether anaesthesia and gastric tissues were collected for morphological and biochemical studies. Subcutaneously administered pharmacological dose of adrenaline bitartrate caused oxidative stress inducing gastric lesion in male albino rats as evident from the altered levels of biomarkers of oxidative stress, activities of antioxidant and mitochondrial enzymes related to energy metabolism with changes in tissue morphology. Pre-treatment of rats with oleic acid dose-dependently protected against these gastric injuries induced by adrenaline indicating the potentiality of oleic acid in protecting against adrenaline induced gastric injury in male albino rats where antioxidant mechanisms appear to play a pivotal role in mediating such protection.
