Química Nova (Jan 2008)

Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS

  • Sérgio Luiz Dalmora,
  • Liberato Brum Junior,
  • Ricardo Machado Ferretto,
  • Paulo Renato de Oliveira,
  • Thiago Barth,
  • Maximiliano da Silva Sangoi

DOI
https://doi.org/10.1590/S0100-40422008000300021
Journal volume & issue
Vol. 31, no. 3
pp. 574 – 578

Abstract

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A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solid-phase extraction. The method was performed on a Luna C18 column and the mobile phase consisted of acetonitrile:water (95:5, v/v)/ammonium acetate (pH 4.0; 10 mM), run at a flow rate of 0.6 mL/min. The method was linear in the range of 1-5000 ng/mL (r²>0.99). The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%. Moreover, method validation demonstrated acceptable results for the precision, accuracy and stability studies.

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