ASSOCIATION OF LETHAL OUTCOMES IN SURGICAL SEPSIS WITH ALLELIC VARIANTS OF TNF Α AND HEAT SHOCK PROTEIN (HSP70-2) GENES

Abstract

Abstract. Taking into account close localization of genes encoding HSP70-2 and TNFα and mutual influence of their products upon development of septic process, we investigated allelic polymorphism in the coding region of HSP70-2 gene at position 1267, and a promoter polymorphism of TNFα gene (–308 position) in sixty-one patients with surgical sepsis, as compared with 100 healthy donors, assuming that certain genomic variants can be associated with susceptibility for sepsis and/or fatal outcomes in severe sepsis. Significant differences in frequencies of HSP70-2 gene alleles have been found in the group with sepsis against healthy controls. Prevalence of HSP70-2*B/B allele is increased in the group of patients with sepsis, and a predisposal for sepsis development is shown for carriers of the given genotype. When analyzing carriers of combined HSP70-2/TNFα allelic variants among dead patients vs survivors, we have revealed specific combinations that are detected solely among non-survived patients, i.e., HSP70-2 A/TNFα AA, or only among survivors (HSP70-2BB /TNFα AA и HSP70-2 BB/TNFα AG). The results obtained allow us to suggest about certain genetic factors that may sufficiently influence intensity of inflammation in sepsis and, hence, upon clinical manifestations and outcomes of the disease.

Keywords

• tnfα
• hsp70-2
• surgical sepsis