The positive effect of eugenol on acute pancreatic tissue injury: a rat experimental model

Abstract

INTRODUCTION: We present a rat experimental model used to evaluate the possible reduction in the extent of pancreatic tissue injury in acute pancreatitis cases, after administration of eugenol. METHODS: one twenty Wistar rats were used, which were randomly assigned in 3 groups: sham (n=20), control (n=50) and eugenol (n=50). Acute pancreatitis was induced by biliopancreatic ligation in the control and eugenol groups, but not in the Sham group. In the eugenol group, eugenol was administered per-os. Five histopathological parameters, such as edema, inflammatory infiltration, duct dilatation, hemorrhage and acinar necrosis were evaluated. RESULTS: at 72 h from acute pancreatitis induction, the total histological score was diminished in the eugenol group (p=0.0005) and duct dilatation and inflammatory infiltration were reduced compared to the control group(p=0.05). In addition, at 72 h, eugenol reduced pancreatic myeloperoxidase activity (p=0.0005). CONCLUSION: eugenol, a highly free radical scavenger agent, may have a preventive role in acute pancreatic injury, as it was evident in our rat experimental model.

Keywords

• acute pancreatitis
• eugenol
• histopathological evaluation
• immunohistochemistry
• rat experimental model