Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ (Feb 2021)
The Effect of Alcoholic Extract of Rhizomes of Greater Galangal (Alpinia galanga L.) on the Serum Antioxidant Enzymes for Japanese Quail During Oxidative Stress Induced by Hydrogen Peroxide
Abstract
This study aimed to investigate the effect of alcoholic extract of greater galangal rhizomes on the serum antioxidant enzymes level in Japanese quail during oxidative stress induced by hydrogen peroxide. Two hundred and sixteen, 7 days-old of Japanese quail birds were randomly distributed into four groups (n=54) with three replicates per group and 18 chicks per replicate. The groups as follows: the first group was drank water without any addition as control. The second group was supplied with 4 ml.-1 hydrogen peroxide H2O2 (40%). The third group was supplied with 4 ml.-1 both hydrogen peroxide (40%) and alcoholic extract of greater galangal rhizomes. The fourth group was supplied with 4 ml.-1 alcoholic extract of greater galangal rhizomes. The results showed the lowest significant (P<0.05) decrease of malonaldehyde (MDA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations in the fourth group while, the highest significant (P<0.05) increase was recorded in the second group. The highest significant (P<0.05) activity level of superoxide dismutase (SOD) and glutathione peroxidase (GPx) recorded in the fourth group, on the other hand, it was significantly lowest in the second group. The results explained there is no significant difference (P<0.05) in the concentration of MDA,SOD, GPx AST and ALT between the first (control) and third groups. The results indicated there is no significant differs between males and females in the MDA,SOD, GPx AST and ALT. It can be concluded, oxidative stress was caused a negative impacts on serum SOD, GPx. AST and ALT enzymes. Moreover, it was caused an increase in MDA levels. The alcoholic extract of rhizomes of greater galangal was reduced and reversed the H2O2 impacts. All parameters non-significantly influenced by sexes.
Keywords