Pathogens (Aug 2021)

Pragmatic Strategy for Fecal Specimen Storage and the Corresponding Test Methods for <i>Clostridioides difficile</i> Diagnosis

  • Seong Won Nho,
  • Minjae Kim,
  • Seong-Jae Kim,
  • Steven L. Foley,
  • Rajesh Nayak,
  • Ohgew Kweon,
  • Carl E. Cerniglia

DOI
https://doi.org/10.3390/pathogens10081049
Journal volume & issue
Vol. 10, no. 8
p. 1049

Abstract

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The quality of fecal specimens is one of the factors responsible for successful Clostridioides difficile infection (CDI) diagnosis. The quality depends largely on the storage conditions, including the temperature and time period. In this study, we organized the outputs of previous studies, filled experimental gaps in the knowledge of storage conditions, and introduced a pragmatic strategy for fecal storage for CDI diagnosis. A 5-step pathway was adopted to develop the fecal specimen storage strategy as follows: step 1, bibliomic analysis; step 2, experimental gap-filling; step 3, comparative evaluation; step 4, strategy development; step 5, internal review. Step 1 identified eight articles providing experimental information on the effects of fecal specimen storage conditions on the effectiveness of C. difficile detection methods. Step 2 provided additional quantitative data on C. difficile vegetative and spore cell viability and DNA stability. All previous and current results were compared (step 3). In step 4, fir general and nine special strategies were developed, followed by an internal review of the overall approaches (step 5). It is recommended to separate fecal samples into aliquots before testing and storing them. It is particularly recommended that fecal specimen samples be stored for CDI diagnosis at 4 °C for up to 60 days for all test methods.

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