A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV

Hung-Cheng Chang; Yu-Tin Chao; Jia-Yush Yen; Ya-Lin Yu; Chun-Nan Lee; Bing-Ching Ho; Kou-Chen Liu; Jiunn Fang; Chii-Wann Lin; Jiun-Haw Lee

doi:10.1155/2015/306708

Advances in Materials Science and Engineering (Jan 2015)

A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV

Hung-Cheng Chang,
Yu-Tin Chao,
Jia-Yush Yen,
Ya-Lin Yu,
Chun-Nan Lee,
Bing-Ching Ho,
Kou-Chen Liu,
Jiunn Fang,
Chii-Wann Lin,
Jiun-Haw Lee

Affiliations

Hung-Cheng Chang: Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan
Yu-Tin Chao: Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan
Jia-Yush Yen: Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan
Ya-Lin Yu: Department of Mechanical Engineering, National Taiwan University, Taipei 106, Taiwan
Chun-Nan Lee: College of Medicine, National Taiwan University, Taipei 106, Taiwan
Bing-Ching Ho: College of Medicine, National Taiwan University, Taipei 106, Taiwan
Kou-Chen Liu: Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan
Jiunn Fang: Department of Aerospace and System Engineering, Feng Chia University, Taichung 407, Taiwan
Chii-Wann Lin: Institute of Biomedical Engineering, National Taiwan University, Taipei 106, Taiwan
Jiun-Haw Lee: Department of Electronic Engineering, National Taiwan University, Taipei 106, Taiwan

DOI: https://doi.org/10.1155/2015/306708
Journal volume & issue: Vol. 2015

Abstract

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This paper presents a LAMP- (loop-mediated isothermal amplification-) based lab-on-disk optical system that allows the simultaneous detection of hepatitis B virus, hepatitis C virus, and cytomegalovirus. The various flow stages are controlled in the proposed system using different balance among centrifugal pumping, Coriolis pumping, and the capillary force. We have implemented a servo system for positioning and speed control for the heating and centrifugal pumping. We have also successfully employed a polymer light-emitting diode section for turbidity detection. The easy-to-use one-click system can perform diagnostics in less than 1 hour.

Published in Advances in Materials Science and Engineering

ISSN: 1687-8434 (Print); 1687-8442 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.hindawi.com/journals/amse/

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