Nature Communications (Jun 2017)

Optogenetic protein clustering through fluorescent protein tagging and extension of CRY2

  • Hyerim Park,
  • Na Yeon Kim,
  • Sangkyu Lee,
  • Nury Kim,
  • Jihoon Kim,
  • Won Do Heo

DOI
https://doi.org/10.1038/s41467-017-00060-2
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 8

Abstract

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Cryptochrome 2 (CRY2) from A. thaliana can be used to control light-dependent protein homo-oligomerization, but the molecular mechanism of CRY2 clustering is not known, limiting its application. Here the authors identify determinants of CRY2 clustering and engineer fusion partners to modulate clustering efficiency.