Induced pluripotent stem cell line heterozygous for p.R501X mutation in filaggrin: KCLi003-A

Nikola Kolundzic; Preeti Khurana; Liani Devito; Jakob Jeriha; Jennifer Marx; Carl Hobbs; Xuan Fei Colin Cornelius Wong; John E.A. Common; Mirjana Liovic; Sandrine Dubrac; Robert Gruber; Matthias Schmuth; Theodora M. Mauro; Dusko Ilic

Stem Cell Research (Aug 2019)

Induced pluripotent stem cell line heterozygous for p.R501X mutation in filaggrin: KCLi003-A

Nikola Kolundzic,
Preeti Khurana,
Liani Devito,
Jakob Jeriha,
Jennifer Marx,
Carl Hobbs,
Xuan Fei Colin Cornelius Wong,
John E.A. Common,
Mirjana Liovic,
Sandrine Dubrac,
Robert Gruber,
Matthias Schmuth,
Theodora M. Mauro,
Dusko Ilic

Affiliations

Nikola Kolundzic: Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK
Preeti Khurana: Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK
Liani Devito: Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK
Jakob Jeriha: Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Jennifer Marx: Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK
Carl Hobbs: Histology Laboratory, Wolfson Centre for Age-Related Diseases, School of Biomedical Sciences, King's College London, London, UK
Xuan Fei Colin Cornelius Wong: Skin Research Institute of Singapore, A*STAR, Singapore, Singapore
John E.A. Common: Skin Research Institute of Singapore, A*STAR, Singapore, Singapore
Mirjana Liovic: Medical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia
Sandrine Dubrac: Department of Dermatology, Venereology and Allergology, Medical University of Innsbruck, Innsbruck, Austria
Robert Gruber: Department of Dermatology, Venereology and Allergology, Medical University of Innsbruck, Innsbruck, Austria
Matthias Schmuth: Department of Dermatology, Venereology and Allergology, Medical University of Innsbruck, Innsbruck, Austria
Theodora M. Mauro: Dermatology Services, Veteran Affairs Medical Center, University of California San Francisco, San Francisco, CA, USA
Dusko Ilic: Stem Cell Laboratory, Department of Women and Children's Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK; Assisted Conception Unit, Guy's Hospital, London, UK; Corresponding author at: Assisted Conception Unit, Guy's Hospital, 11th Fl. Tower Wing, London SE1 9RT, UK.

Journal volume & issue: Vol. 39

Abstract

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We have generated an induced pluripotent stem cell (iPSC) line KCLi003-A (iOP101) from epidermal keratinocytes of a female donor, heterozygous for the loss-of-function mutation p.R501X in the filaggrin gene (FLG), using non-integrating Sendai virus vectors. Derivation and expansion of iPSCs were performed under xeno-free culture conditions. Characterization and validation of KCLi003-A line included molecular karyotyping, mutation screening using restriction enzyme digestion, next generation sequencing (NGS), while pluripotency and differentiation potential were confirmed by expression of associated markers in vitro and by in vivo teratoma assay.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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